Cancelled 216403a Seminar

WiSe 17/18: Protein Analysis and Microsequencing / Proteinanalytik und Mikrosequenzierung

Christoph Weise, Werner Schröder

Information for students

Tombola: Mon., 16.10.17, 9:00; lecture hall Biochemie (Lise-Meitner-Hörsaal), Thielallee 63
ATTENTION: students can not enroll themselves for this module in CM. The enrollment will be organised by the student's office after the tombola. close

Additional information / Pre-requisites

Seminar and practical course (216403a and b) can be selected:
  • in the main study period (5 SWS);
  • in the biochemistry master's method module "special methods of molecular biology (5 LP)".

The seminar 216403a can be selected in the module "special aspects of molecular biology" in combination with another seminar. close


Inhalt / contents

purification of proteins by 1 and 2D electrophoresis, RP and IE high performance liquid chromatographie, electroblotting of proteins onto PVDF membrane, manual and automatic N-terminal sequencing of proteins and peptides (ABI 473A Proteinsequencer), peptide generation by enzymatic and chemical cleavage methods ( in solution, onto PVDF membrane and in gel) modification and identification of cysteine and phosphoserine residues in proteins amino acid analysis, database search concerning protein identification and homology automatic synthesis of DNA and RNA oligomers, purification by HPLC and gel- electrophoresis, theoretical: mass spectrometry in protein and nucleic acid analysis lectures: advanced methods in protein sequence analysis

Dr. C. Weise:
Dr. W. Schröder: close

Subjects A - Z