216461a
Seminar
WiSe 17/18: Production and Biophysical Analysis of Selected Membrane Proteins Part 1 / Produktion und biophysikalische Analyse ausgewählter Membranproteine Teil 1
Ramona Schlesinger
Comments
Content of the seminar:
- Membrane proteins with focus on different classes of retinal proteins and their functionality
- Different kinds of membrane proteins expression systems and especially those of the model proteins in the course (Escherichia coli, Pichia pastoris)
- Biomembranes, nanodiscs, lipids, detergents
- Different methods for directed mutagenesis via PCR
- Protein purification procedures like chromatographies, selective precipitation, gradient and sequential centrifugation etc. with practical tips for the lab
- Crystallisation of membrane proteins
- selected topics e.g. membrane protein folding, interaction of (membrane) proteins, stalled ribosomes, antibiotics
- Research projects in AG Schlesinger