216641c
Laboratory Course
WiSe 18/19: Selbstreplizierende Proteinpartikel und Neurodegeneration bei Morbus Alzheimer, Parkinson-Krankheit und Prionerkrankungen / Self-replicating protein particles and neurodegeneration in Alzheimer´s disease, Parkinson´s disease and prion diseases
Michael Beekes
Information for students
Practical course with seminar.
Prerequisite: Attendance of lecture 216641a.
Slots will be distributed by the lecturer to selected students, which already successfully attended the lecture in a prior semester.
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Additional information / Pre-requisites
Briefing: The breifing will take place during the lecture 216641 a in the summer semester 2017
Comments
Content:
PD Dr. M. Beekes: BeekesM@rki.de close
- Preparation of paraffin-embedded brain sections for histological and immunohistochemical analyses
- Immunohistochemical detection of Parkinson-associated aggregated alpha-synuclein in brain sections
- Immunohistochemical detection of glial fibrillary acid protein as a marker for astrocytes in brain sections
- Immunohistochemical detection of neuron-specific nuclear protein as a marker for neurons in brain sections
- Immunohistochemical detection of dopaminergic neurons in brain sections
- Haematoxylin eosin staining of brain sections for visualisation of vacuolar prion pathology
- Detection of infectious prion protein (PrPSc) in Proteinase K - digested brain sections on nitrocellulose membranes by the paraffin-embedded tissue (PET) blot technique
- Western blot detection of Alzheimer-associated aggregated amyloid-beta (Aß) and tau protein from brain tissue
- Extraction, purification and Western blot detection of infectious prion protein (PrPSc) from prion-infected muscle tissue
- In vitro analysis of the chemical destabilisation and degradation of infectious prion protein (PrPSc)
- Cell-free protein misfolding cyclic amplification (PMCA) of infectious prion proteins
PD Dr. M. Beekes: BeekesM@rki.de close