205191
Practice seminar
SoSe 13: Production of biological samples in biophysics
Ramona Schlesinger
Comments
Blockkurs vom 17.-21.09.2012, täglich von 9-18 Uhr, Anmeldung bis zum 31.07.2012 Email: r.schlesinger@fu-berlin.de
6 Teilnehmer
Practical exercises:
-molecular biological techniques e.g. transformation of bacteria with plasmids in preparation of expressing a membrane protein
-cultivation of bacteria to express the protein
-purification of membrane proteins by affinity chromatography
-amplification of DNA by PCR (polymerase chain reaction)
-analysis of DNA with enzymes and agarose-gel-electrophoresis and analysis of proteins on SDS-gels.
Zielgruppe
Master students, diploma strudents and PhD students with interest in generating their biological samples by themselves for biophysical investigations close