205400
Lecture
SoSe 14: site-directed spin labeling EPR
Enrica Bordignon
Comments
The course gives an introduction to electron paramagnetic resonance (EPR) spectroscopy, with special focus on site-directed spin labeling (SDSL) for the study of biomolecules. SDSL EPR is a biophysical technique providing structural and dynamic information on proteins to bridge the gap between the static snapshot of a crystal structure and the protein's dynamic nature in a physiological environment. The course starts from a general description of the magnetic resonance phenomena: classical description and Bloch equations, quantum description of the spin system, spin Hamiltonian, density operators, product operator formalism (spin 1/2). The nitroxide-based spin labels available for protein studies are presented and the properties of their continuous wave EPR spectra are described in detail with focus on the dynamics and polarity effects on the spectral features and the multi-frequency advantage. Continuous wave and pulsed methods to measure interspin distances are described. An introduction to the continuous wave and pulsed spectrometers is also given. State of the art applications of SDSL EPR to water soluble and membrane proteins are presented.
Exercises will cover the theoretical aspects presented in the course and a lab course may be offered (to be decided with the students) on continuous wave or pulsed EPR techniques. close
12 Class schedule
Regular appointments
Mon, 2014-04-14 16:00 - 18:00
Mon, 2014-04-28 16:00 - 18:00
Mon, 2014-05-05 16:00 - 18:00
Mon, 2014-05-12 16:00 - 18:00
Mon, 2014-05-19 16:00 - 18:00
Mon, 2014-05-26 16:00 - 18:00
Mon, 2014-06-02 16:00 - 18:00
Mon, 2014-06-16 16:00 - 18:00
Mon, 2014-06-23 16:00 - 18:00
Mon, 2014-06-30 16:00 - 18:00
Mon, 2014-07-07 16:00 - 18:00
Mon, 2014-07-14 16:00 - 18:00