216451a
Seminar
SoSe 18: Membrane Protein Expression in Cell-Free Systems / Zellfreie Synthese von Membranproteinen
Stefan Kubick
Information for students
Seminar kann nicht einzeln besucht werden!
Additional information / Pre-requisites
Attention!: Participation in the lecture and seminar 216501 a, b in an earlier semester are prerequesites for the enrollment in this course!
Comments
Content
Cell-free Protein Synthesis in pro- and eukaryotic Systems
Cell-free Protein Synthesis in pharmaceutical Protein Research and Production
Cell-free coupled and linked transcription/translation Systems
Batch-formatted Reactions and Dialysis (CECF)-Systems
High-Throughput Membrane Protein Expression using Cell-free Systems
Cell-free Synthesis of toxic Proteins, labelled Proteins and Protein Conjugates
Cell-free Synthesis of “difficult-to-express” Proteins
Cell-free Antibody-Production
Chaperones, Nanodiscs and Detergents in Cell-free Systems
Qualitative and quantitative Analysis of cell-free produced Membrane Proteins
(Autoradiography and hot TCA Precipitation)
posttranslational Modification Pathways in Cell-free Systems
Generation of DNA-Templates by Expression-PCR
Co-translational Translocation in eukaryotic Cell-free Systems
Effects of Signal-Sequences in eukaryotic Cell-free Systems
Functional Analysis of Cell-free produced Ionchannels and GPCRs
Preparation of Giant Unilamelar Vesicles (GUVs)
Single-Molecule-Analysis of membrane-embedded Proteins
In vitro Synthesis of Glycoproteins
Site-specific Incorporation of non-canonical Amino Acids
Orthogonal Systems
Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de close
Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de close