216621b
Laboratory Course
SoSe 18: Analyzing musculoskeletal development in vivo / In vivo Analyse des muskuloskeletalen Systems
Sigmar Stricker
Information for students
ATTENTION: The absolvation of the modul 216701 "Introduction into delevelopmental biology" in an earlier semester is a prerequisite for the participation in the method course
Attendance of the seminar is obligatory for the attendance of the practical course close
Attendance of the seminar is obligatory for the attendance of the practical course close
Comments
Content:
Prof. Dr. S. Stricker: sigmar.stricker@fu-berlin.de close
- Dissection of laboratory mice
- Tissue embedding, cryoconservation
- Cryosectioning of tissue
- Histological methods; Hematoxylin/Eosin staining, Oil Red O staining of tissue sections
- Alizarin red / Alcian blue staining of skeletal preparations
- PCR-Genotyping of genetically modified laboratory mice
- Immunohistochemistry
- mRNA in-situ hybridization
- imaging of whole-embryo specimen
- imaging of histological sections
- imaging of fluorescently labelled tissue sections
Prof. Dr. S. Stricker: sigmar.stricker@fu-berlin.de close