216451b
Laboratory Course
SoSe 18: Membrane Protein Expression in Cell-Free Systems / Zellfreie Synthese von Membranproteinen
Stefan Kubick
Information for students
ACHTUNG: Dieses Semester wird das Praktikum an zwei Terminen angeboten. Die Termine werden unabhängig verlost. 10 Studierende können vom 03.09. bis 14.09.18 Praktikum und Seminar belegen.
Zwei weitere Studierende können Praktikum und Seminar vom 11.06. - 22.06.18 belegen. close
Additional information / Pre-requisites
Attention!: Participation in the lecture and seminar 216501 a, b in an earlier semester are prerequesites for the enrollment in this course!
Comments
Content:
The practical course “cell-free synthesis of membrane proteins” provides an overview on the production of functional membrane proteins. Methods for the generation of DNA- and RNA-templates suitable for transcription and translation in cell-free systems will be emphasized, with reference to prokaryotic and eukaryotic in vitro translation systems. The course covers specific case studies on “difficult-to-express” proteins. Labeling of membrane proteins by the incorporation of non-canonical amino acids will be explored in detail and the course concludes with a “hands-on-training” in the preparation of glycoproteins and posttranslationally modified membrane proteins in novel cell-free systems. This course includes pdfs to each segment.
Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de close
The practical course “cell-free synthesis of membrane proteins” provides an overview on the production of functional membrane proteins. Methods for the generation of DNA- and RNA-templates suitable for transcription and translation in cell-free systems will be emphasized, with reference to prokaryotic and eukaryotic in vitro translation systems. The course covers specific case studies on “difficult-to-express” proteins. Labeling of membrane proteins by the incorporation of non-canonical amino acids will be explored in detail and the course concludes with a “hands-on-training” in the preparation of glycoproteins and posttranslationally modified membrane proteins in novel cell-free systems. This course includes pdfs to each segment.
Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de close