216403a Seminar

SoSe 19: Protein Analysis and Microsequencing / Proteinanalytik und Mikrosequenzierung

Christoph Weise, Werner Schröder

Hinweise für Studierende


Inhalt / contents

  • Protein purification (1D- and 2D-PAGE, RP-HPLC, IEX, SEC)
  • Electro-blotting and electro-elution from gels
  • Protein fragmentation by enzymatic and chemical methods (in solution, in gel and on membrane)
  • Amino-acid analysis, derivatization and identification of amino acids
  • Manual and automated N-terminal sequencing of proteins and peptides (Edman degradation)
  • C-terminal analysis of proteins and peptides
  • Database searches and protein sequence analysis
  • Automated synthesis of DNA and RNA oligomers, double-strand analysis
theoretical: mass spectrometry in protein and nucleic acid analysis
lectures: advanced methods in protein sequence analysis

Dr. C. Weise: chris.weise@biochemie.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de Schließen

Studienfächer A-Z