216451a        
        
          Seminar        
      
      SoSe 20: Zellfreie Synthese von Membranproteinen
Stefan Kubick
Hinweise für Studierende
        Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, findet diese Lehrveranstaltung vorerst nicht statt. Sie wird stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  
This course cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again.
 
Seminar kann nicht einzeln besucht werden! Schließen
  This course cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again.
Seminar kann nicht einzeln besucht werden! Schließen
Zusätzl. Angaben / Voraussetzungen
       Achtung!: Voraussetzung ist der Besuch von V/S 216501 a, b in einem vorangegangenen Semester.          
  Kommentar
        Content 
 Cell-free Protein Synthesis in pro- and eukaryotic Systems  
 Cell-free Protein Synthesis in pharmaceutical Protein Research and Production  
 Cell-free coupled and linked transcription/translation Systems  
 Batch-formatted Reactions and Dialysis (CECF)-Systems  
 High-Throughput Membrane Protein Expression using Cell-free Systems  
 Cell-free Synthesis of toxic Proteins, labelled Proteins and Protein Conjugates  
 Cell-free Synthesis of "difficult-to-express" Proteins  
 Cell-free Antibody-Production  
 Chaperones, Nanodiscs and Detergents in Cell-free Systems  
 Qualitative and quantitative Analysis of cell-free produced Membrane Proteins  
 (Autoradiography and hot TCA Precipitation)  
 posttranslational Modification Pathways in Cell-free Systems  
 Generation of DNA-Templates by Expression-PCR  
 Co-translational Translocation in eukaryotic Cell-free Systems  
 Effects of Signal-Sequences in eukaryotic Cell-free Systems  
 Functional Analysis of Cell-free produced Ionchannels and GPCRs  
 Preparation of Giant Unilamelar Vesicles (GUVs)  
 Single-Molecule-Analysis of membrane-embedded Proteins  
 In vitro Synthesis of Glycoproteins  
 Site-specific Incorporation of non-canonical Amino Acids  
 Orthogonal Systems  
Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de Schließen
  Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de Schließen
