Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, findet diese Lehrveranstaltung vorerst nicht statt. Sie werden wieder stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  
This courss cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again.

Schließen

Inhalt / contents

• Protein separation by 1D and 2D-PAGE
• Electro-blotting of proteins onto PVDF membrane
• Electro-elution and crush-and-soak extraction of proteins from gels
• Generation of peptides by enzymatic and chemical cleavage (in solution, in gel and on membrane)
• Derivatization and identification of cysteine and phosphoserine residues in proteins
• Performic acid oxidation
• Amino-acid analysis (total acid hydrolysis of proteins)
• Peptide separation by RP-HPLC
• Desalting of proteins and peptides by HPLC and SEC
• Manual N-terminal sequencing of proteins (PITC, ladder sequencing)
• Mass-spectrometric analysis of proteins and peptides
• Database search concerning protein identification and homology
• Sequence analysis (secondary structure prediction, Chou & Fasman, and hydropathy plot, Kyte & Doolittle)
• Automatic synthesis of DNA and RNA oligomers and their purification by HPLC and gel electrophoresis

theoretical: mass spectrometry in protein and nucleic acid analysis
lectures: advanced methods in protein sequence analysis

Dr. C. Weise: chris.weise@biochemie.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de Schließen