216451a Seminar

SoSe 20: Membrane Protein Expression in Cell-Free Systems

Stefan Kubick

Information for students

Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, findet diese Lehrveranstaltung vorerst nicht statt. Sie wird stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  

This course cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again. 

Seminar kann nicht einzeln besucht werden! close

Additional information / Pre-requisites

Attention!: Participation in the lecture and seminar 216501 a, b in an earlier semester are prerequesites for the enrollment in this course!

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Content
  • Cell-free Protein Synthesis in pro- and eukaryotic Systems
  • Cell-free Protein Synthesis in pharmaceutical Protein Research and Production
  • Cell-free coupled and linked transcription/translation Systems
  • Batch-formatted Reactions and Dialysis (CECF)-Systems
  • High-Throughput Membrane Protein Expression using Cell-free Systems
  • Cell-free Synthesis of toxic Proteins, labelled Proteins and Protein Conjugates
  • Cell-free Synthesis of “difficult-to-express” Proteins
  • Cell-free Antibody-Production
  • Chaperones, Nanodiscs and Detergents in Cell-free Systems
  • Qualitative and quantitative Analysis of cell-free produced Membrane Proteins
  • (Autoradiography and hot TCA Precipitation)
  • posttranslational Modification Pathways in Cell-free Systems
  • Generation of DNA-Templates by Expression-PCR
  • Co-translational Translocation in eukaryotic Cell-free Systems
  • Effects of Signal-Sequences in eukaryotic Cell-free Systems
  • Functional Analysis of Cell-free produced Ionchannels and GPCRs
  • Preparation of Giant Unilamelar Vesicles (GUVs)
  • Single-Molecule-Analysis of membrane-embedded Proteins
  • In vitro Synthesis of Glycoproteins
  • Site-specific Incorporation of non-canonical Amino Acids
  • Orthogonal Systems

  • Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de close

    Subjects A - Z