Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, findet diese Lehrveranstaltung vorerst nicht statt. Sie wird stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  

This course cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again. 

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Content

Cell-free Protein Synthesis in pro- and eukaryotic Systems

Cell-free Protein Synthesis in pharmaceutical Protein Research and Production

Cell-free coupled and linked transcription/translation Systems

Batch-formatted Reactions and Dialysis (CECF)-Systems

High-Throughput Membrane Protein Expression using Cell-free Systems

Cell-free Synthesis of toxic Proteins, labelled Proteins and Protein Conjugates

Cell-free Synthesis of “difficult-to-express” Proteins

Cell-free Antibody-Production

Chaperones, Nanodiscs and Detergents in Cell-free Systems

Qualitative and quantitative Analysis of cell-free produced Membrane Proteins

(Autoradiography and hot TCA Precipitation)

posttranslational Modification Pathways in Cell-free Systems

Generation of DNA-Templates by Expression-PCR

Co-translational Translocation in eukaryotic Cell-free Systems

Effects of Signal-Sequences in eukaryotic Cell-free Systems

Functional Analysis of Cell-free produced Ionchannels and GPCRs

Preparation of Giant Unilamelar Vesicles (GUVs)

Single-Molecule-Analysis of membrane-embedded Proteins

In vitro Synthesis of Glycoproteins

Site-specific Incorporation of non-canonical Amino Acids

Orthogonal Systems

Dr. S. Kubick: stefan.kubick@izi-bb.fraunhofer.de close