Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, findet diese Lehrveranstaltung vorerst nicht statt. Sie wird stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind. This course cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again. close

Practical content:

• Studying BMP/TGFß- induced signal transduction on multiple levels of the signaling cascade
• Use of biochemical assays which allow to analyze BMP/TGFß signal transduction in a quantitative manner (qRT-PCR, quantitative Western-Blotting)
• Use of biochemical assays which allow for analysis of BMP/TGFß signal transduction in a kinetic manner. Ligand stimulation, transcription factor translocation events, use of Luciferase-based reporter gene assay, activation of BMP-SMAD target genes
• Use of biochemical assays which allow to analyze BMP/TGFß signal transduction with spatial resolution, subcellular localization of transcription factors using fluorescence microscopy
• Using tools/strategies to artificially manipulate signal transduction (small molecule inhibitors targeting receptor-kinase activity, overexpression of tagged proteins of the BMP signal transduction cascade by transient-transfection methodology of overexpression plasmids.
• Extensive discussion on experimental design, drawbacks and pitfalls when designing an experiment to investigate signal transduction.
• Extensive discussions on appropriate controls to be implemented into the experimental design.
• Summary of the experimental results by simulating how to write a primary research paper.

Prof. Dr. P. Knaus: knaus@chemie.fu-berlin.de
Dr. Christian Hiepen: christian.hiepen@fu-berlin.de close