216404b Laboratory Course

SoSe 20: Bioanalytical Mass Spectrometry / Proteomic Analysis

Christoph Weise

Information for students

Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, finden Praktika vorerst nicht statt. Sie werden wieder stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  
The lab courses cannot take place at the moment, as any courses that require attendance are prohibited. They will take place, when courses that require attendance are permitted again.

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Additional information / Pre-requisites

Inhalt / Content:
  • Sample preparation techniques for ESI and MALDI mass spectrometry (MS)
  • In-gel / in-solution digest
  • MALDI peptide mass fingerprinting
  • Recording and interpretation of MALDI MS/MS and ISD spectra
  • Mass determination of intact proteins
  • LC-ESI-MS
  • Quantitative proteomics (data analysis)
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Comments

Inhalt / Content:
  • Sample preparation techniques for ESI and MALDI mass spectrometry (MS)
  • In-gel / in-solution digest
  • MALDI peptide mass fingerprinting
  • Recording and interpretation of MALDI MS/MS and ISD spectra
  • Mass determination of intact proteins
  • LC-ESI-MS
  • Quantitative proteomics (data analysis)
chris.weise@biochemie.fu-berlin.de close

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