216621b Laboratory Course

SoSe 20: Analyzing musculoskeletal development in vivo

Sigmar Stricker

Information for students

Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, finden Praktika vorerst nicht statt. Sie werden wieder stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  
The lab courses cannot take place at the moment, as any courses that require attendance are prohibited. They will take place, when courses that require attendance are permitted again.

ATTENTION: The absolvation of the modul 216701 "Introduction into delevelopmental biology" in an earlier semester is a prerequisite for the participation in the method course
Attendance of the seminar is obligatory for the attendance of the practical course close

Comments

Content:
  • Dissection of laboratory mice
  • Tissue embedding, cryoconservation
  • Cryosectioning of tissue
  • Histological methods; Hematoxylin/Eosin staining, Oil Red O staining of tissue sections
  • Alizarin red / Alcian blue staining of skeletal preparations
  • PCR-Genotyping of genetically modified laboratory mice
  • Immunohistochemistry
  • mRNA in-situ hybridization
  • imaging of whole-embryo specimen
  • imaging of histological sections
  • imaging of fluorescently labelled tissue sections


Prof. Dr. S. Stricker: sigmar.stricker@fu-berlin.de close

Subjects A - Z