216406a
Seminar
SoSe 20: Gene editing with CRISPR/Cas9 for cell biology
Francesca Bottanelli
Information for students
Powerpoint with voice-over and/or videoconference at the regular time.
Comments
Content:
The students will learn:
• How to use bio-informatic tools for CRISPR/Cas9 technology
• How to clone all the required plasmids for the insertion of tags into the genomic locus of genes of interest
• How to culture and transfect human cell culture models
• Live-cell confocal and stimulated-emission depletion (STED) microscopy
Prof. Dr. Bottanelli: Francesca.bottanelli@fu-berlin.de close
The students will learn:
• How to use bio-informatic tools for CRISPR/Cas9 technology
• How to clone all the required plasmids for the insertion of tags into the genomic locus of genes of interest
• How to culture and transfect human cell culture models
• Live-cell confocal and stimulated-emission depletion (STED) microscopy
Prof. Dr. Bottanelli: Francesca.bottanelli@fu-berlin.de close