Da aus aktuellem Anlass momentan keine Präsenzlehrveranstaltungen erlaubt sind, findet diese Lehrveranstaltung vorerst nicht statt. Sie werden wieder stattfinden, wenn Kurse, die eine Teilnahme erfordern, wieder erlaubt sind.  
This courss cannot take place at the moment, as any courses that require attendance are prohibited. It will take place, when courses that require attendance are permitted again.

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Inhalt / contents

• Protein purification (1D- and 2D-PAGE, RP-HPLC, IEX, SEC)
• Electro-blotting and electro-elution from gels
• Protein fragmentation by enzymatic and chemical methods (in solution, in gel and on membrane)
• Amino-acid analysis, derivatization and identification of amino acids
• Manual and automated N-terminal sequencing of proteins and peptides (Edman degradation)
• C-terminal analysis of proteins and peptides
• Database searches and protein sequence analysis
• Automated synthesis of DNA and RNA oligomers, double-strand analysis

theoretical: mass spectrometry in protein and nucleic acid analysis
lectures: advanced methods in protein sequence analysis

Dr. C. Weise: chris.weise@biochemie.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de close