216403a
Seminar
SoSe 21: Protein Analysis and Microsequencing
Christoph Weise, Werner Schröder
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Inhalt / contents
lectures: advanced methods in protein sequence analysis
Dr. C. Weise: chris.weise@biochemie.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de close
- Protein purification (1D- and 2D-PAGE, RP-HPLC, IEX, SEC)
- Electro-blotting and electro-elution from gels
- Protein fragmentation by enzymatic and chemical methods (in solution, in gel and on membrane)
- Amino-acid analysis, derivatization and identification of amino acids
- Manual and automated N-terminal sequencing of proteins and peptides (Edman degradation)
- C-terminal analysis of proteins and peptides
- Database searches and protein sequence analysis
- Automated synthesis of DNA and RNA oligomers, double-strand analysis
lectures: advanced methods in protein sequence analysis
Dr. C. Weise: chris.weise@biochemie.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de close