204250 Vorlesung

WiSe 12/13: Production of biological samples in biophysics

Ramona Schlesinger

Hinweise für Studierende

Master students, diploma strudents and PhD students with interest in generating their biological samples by themselves for biophysical investigations

Zusätzl. Angaben / Voraussetzungen

Blockkurs vom 04.-08.03.2013, täglich von 9-18 Uhr, Anmeldung bis zum 28.02.2013Schlesinger (max. 6 Plätze)


Lectures about how to mutagenise a protein, cloning techniques, expression and purification of proteins will be given prior to the practical exercises in the lab. Practical exercises : -molecular biological techniques e.g. transformation of bacteria with plasmids in preparation of expressing a membrane protein -cultivation of bacteria to express the protein -purification of membrane proteins by affinity chromatography -analysis of DNA and protein preparations by agarose- and SDS-gelelectrophoresis Schließen

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