Lectures about how to mutagenise a protein, cloning techniques, expression and purification of proteins will be given prior to the practical exercises in the lab. Practical exercises : -molecular biological techniques e.g. transformation of bacteria with plasmids in preparation of expressing a membrane protein -cultivation of bacteria to express the protein -purification of membrane proteins by affinity chromatography -analysis of DNA and protein preparations by agarose- and SDS-gelelectrophoresis Schließen