Cancelled
216403b
Laboratory Course
WiSe 17/18: Protein Analysis and Microsequencing / Proteinanalytik und Mikrosequenzierung
Christoph Weise, Werner Schröder
Information for students
Tombola: Mon., 16.10.17, 9:00; lecture hall Biochemie (Lise-Meitner-Hörsaal), Thielallee 63
ATTENTION: students can not enroll themselves for this module in CM. The enrollment will be organised by the student's office after the tombola. close
ATTENTION: students can not enroll themselves for this module in CM. The enrollment will be organised by the student's office after the tombola. close
Additional information / Pre-requisites
Seminar and practical course (216403a and b) can be selected:
- in the main study period (5 SWS);
- in the biochemistry master's method module "special methods of molecular biology (5 LP)".
The seminar 216403a can be selected in the module "special aspects of molecular biology" in combination with another seminar. close
Comments
Inhalt / contents
purification of proteins by 1 and 2D electrophoresis, RP and IE high
performance
liquid chromatographie, electroblotting of proteins onto PVDF membrane,
manual and automatic N-terminal sequencing of proteins and peptides (ABI
473A Proteinsequencer), peptide generation by enzymatic and chemical cleavage
methods ( in solution, onto PVDF membrane and in gel)
modification and identification of cysteine and phosphoserine residues in
proteins
amino acid analysis, database search concerning protein identification and
homology
automatic synthesis of DNA and RNA oligomers, purification by HPLC and gel-
electrophoresis,
theoretical: mass spectrometry in protein and nucleic acid analysis
lectures: advanced methods in protein sequence analysis
Dr. C. Weise: chris.weise@biochemie.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de close