216502a
Lecture
WiSe 18/19: Macromolecular (protein-protein, protein-DNA/RNA) interactions / Makromoleculare (protein-protein, protein-DNA/RNA) Interaktionen
Sutapa Chakrabarti
Information for students
The number of participants in this block class is limited to 15. Should you wish to participate, please register yourself by sending an e-mail to chakraba@zedat.fu-berlin.de no later than one week before the first lecture.
The class will only be held if at least 6 students are registered on the day before the first lecture! close
The class will only be held if at least 6 students are registered on the day before the first lecture! close
Comments
Inhalt / Content:
Prof. Dr. Sutapa Chakrabarti: chakraba@zedat.fu-berlin.de close
- Thermodynamic and kinetic definitions of macromolecular interactions
- Biochemical methods of determining protein-protein interactions (co-precipitation, size-exclusion analysis, cross-linking mass spectrometry, TAP, FRET, yeast 2-hybrid, proximity-dependent biotin identification)
- Biochemical methods of determining protein nucleic acid interactions (gel-shift assay, protection/foot-printing assays, yeast 3-hybrid)
- Biophysical methods of analysing protein-protein/protein-nucleic acid interactions (analytical ultra-centrifugation, light scattering, isothermal titration calorimetry, surface plasmon resonance, fluorescent anisotropy, microscale thermophoresis)
- Presentation and interpretation of biochemical and biophysical data
- High throughput methods of analysis protein-nucleic acid interactions (micro-array technology, next-generation sequencing based methods such as CLIP and its variations, CRAC, Hits-FLIP and adaptation of classical methods such as SELEX and ChIP to high throughput techniques.
- High throughput methods of analysing protein-protein interactions (computational methods as well as proteomics-based approaches)
Prof. Dr. Sutapa Chakrabarti: chakraba@zedat.fu-berlin.de close