Inhalt/Content:
Computational analysis of next generation sequencing data (mapping, quality control, quantification)
Computational analysis of CRISPR screening data
sgRNA design
Cloning individual sgRNAs
Cloning a pooled sgRNA library with Gibson cloning and estimating its coverage
Quality control of the pooled library by next generation sequencing
Design experiments to validate screen hits with different CRISPR systems
RNA extraction
cDNA generation through reverse transcription
Quantitative PCR
Extraction of genomic DNA
Preparation of amplicon sequencing libraries from genomic DNA


Dr. E. Schulz: edda.schulz@molgen.mpg.de Schließen