216404a
Seminar
WiSe 19/20: Seminar on Bioanalytical Mass Spectrometry / Proteomic Analysis
Christoph Weise
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Inhalt / Content:
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- The proteome (definition, concept)
- Mass spectrometry of biological macromolecules
- Ionisation methods (MALDI and ESI)
- Protein separation by 2D-PAGE, staining methods
- Protein identification via peptide mass fingerprints and MS/MS
- Fragmentation methods and interpretation of MS/MS spectra
- LC-MS / Shotgun proteomics
- LC-MS / Quantitative proteomics, metabolic and chemical labelling techniques
- Targetted proteomics
- MALDI imaging
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