216405b
Laboratory Course
WiSe 20/21: Alternative Splicing and Protein-RNA-Interaction
Florian Heyd
Information for students
Die Teilnahme am Seminar ist Voraussetzung für die Teilnahme am Praktikum.
Comments
Content:
Prof. Dr. F. Heyd: florian.heyd@fu-berlin.de close
- Safe handling of radioisotopes
- Chemical synthesis of oligonucleotides
- Purification of oligonucleotides via HPLC
- Purification of oligonucleotides via PAGE
- Radioactive Labeling of oligonucleotides
- Single-turnover kinetics on hammerhead ribozymes
- Purification of target proteins
- Synthesis of a combinatorial RNA library
- Enrichment of oligoribonucleotides binding to a target
- cDNA synthesis via reverse transcription
- PCR amplification
Prof. Dr. F. Heyd: florian.heyd@fu-berlin.de close