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Laboratory Course
WiSe 20/21: Quantitative Transcriptomics
Andreas Mayer
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Inhalt / contents
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RNA sequencing (RNA-seq) & Subcellular RNA-seq
Mammalian cell culture techniques
Cell counting
State-of-the-art cell fractionation procedures
RNA purification & Quantification
Next-generation sequencing library preparation
Quality control of RNA-seq libraries
Visit of the Max Planck Core sequencing facility (MPI for Molecular Genetics)
RNA-seq data processing
Quality assessment of the RNA-seq data sets
Sequencing read mapping to the human reference genome
Transcript quantification & Data visualization
Dr. Andreas Mayer: mayer@molgen.mpg.de close