216403a Seminar

WiSe 21/22: Protein Analysis and Microsequencing

Jens Peter Fürste, Werner Schröder

Information for students


Inhalt / contents

  • Protein purification (1D- and 2D-PAGE, RP-HPLC, IEX, SEC)
  • Electro-blotting and electro-elution from gels
  • Protein fragmentation by enzymatic and chemical methods (in solution, in gel and on membrane)
  • Amino-acid analysis, derivatization and identification of amino acids
  • Manual and automated N-terminal sequencing of proteins and peptides (Edman degradation)
  • C-terminal analysis of proteins and peptides
  • Database searches and protein sequence analysis
  • Automated synthesis of DNA and RNA oligomers, double-strand analysis
theoretical: mass spectrometry in protein and nucleic acid analysis
lectures: advanced methods in protein sequence analysis

Dr. J.P. Fürste: fuerste@zedat.fu-berlin.de
Dr. W. Schröder: WernerSchroe@zedat.fu-berlin.de close

Subjects A - Z