Cancelled 216641c Laboratory Course

WiSe 21/22: Self-replicating protein particles and neurodegeneration in Alzheimer´s disease, Parkinson´s disease and prion diseases

Michael Beekes

Information for students

Practical course with seminar. Prerequisite: Attendance of lecture 216641a. Slots will be distributed by the lecturer to selected students, which already successfully attended the lecture in a prior semester. close

Additional information / Pre-requisites

Briefing: The briefing will take place during the lecture 216641 a in the summer semester 2019


  • Preparation of paraffin-embedded brain sections for histological and immunohistochemical analyses
  • Immunohistochemical detection of Parkinson-associated aggregated alpha-synuclein in brain sections
  • Immunohistochemical detection of glial fibrillary acid protein as a marker for astrocytes in brain sections
  • Immunohistochemical detection of neuron-specific nuclear protein as a marker for neurons in brain sections
  • Immunohistochemical detection of dopaminergic neurons in brain sections
  • Haematoxylin eosin staining of brain sections for visualisation of vacuolar prion pathology
  • Detection of infectious prion protein (PrPSc) in Proteinase K - digested brain sections on nitrocellulose membranes by the paraffin-embedded tissue (PET) blot technique
  • Western blot detection of Alzheimer-associated aggregated amyloid-beta (Aß) and tau protein from brain tissue
  • Extraction, purification and Western blot detection of infectious prion protein (PrPSc) from prion-infected muscle tissue
  • In vitro analysis of the chemical destabilisation and degradation of infectious prion protein (PrPSc)
  • Cell-free protein misfolding cyclic amplification (PMCA) of infectious prion proteins

PD Dr. M. Beekes: close

Subjects A - Z