216404a Seminar

WiSe 21/22: Seminar on Bioanalytical Mass Spectrometry / Proteomic Analysis

Benno Kuropka, Christoph Weise


Inhalt / Content:
  • The proteome (definition, concept)
  • Mass spectrometry of biological macromolecules
  • Ionisation methods (MALDI and ESI)
  • Protein separation by 2D-PAGE, staining methods
  • Protein identification via peptide mass fingerprints and MS/MS
  • Fragmentation methods and interpretation of MS/MS spectra
  • LC-MS / Shotgun proteomics
  • LC-MS / Quantitative proteomics, metabolic and chemical labelling techniques
  • Targetted proteomics
  • MALDI imaging


Subjects A - Z