216623b Laboratory Course

WiSe 21/22: Funktionelle Genomik mit CRISPR

Edda Schulz


The focus of the course might be shifted towards computational tools and data analysis, if the conditions do not allow extensive lab work at that time.

- Computational analysis of next generation sequencing data (mapping, quality control, quantification)
- Computational analysis of CRISPR screening data
- Data analysis in R
- sgRNA design
- CRISPR screen design
- RNA extraction
- cDNA generation through reverse transcription
- Quantitative PCR
- Flow cytometry
- embryonic stem cell culture
- lentiviral transduction

Dr. E. Schulz: edda.schulz@molgen.mpg.de close

Subjects A - Z