216423b Laboratory Course

WiSe 21/22: Quantitative Transcriptomics

Andreas Mayer


Inhalt / contents

    RNA sequencing (RNA-seq) & Subcellular RNA-seq
    Mammalian cell culture techniques
    Cell counting
    State-of-the-art cell fractionation procedures
    RNA purification & Quantification
    Next-generation sequencing library preparation
    Quality control of RNA-seq libraries
    Visit of the Max Planck Core sequencing facility (MPI for Molecular Genetics)
    RNA-seq data processing
    Quality assessment of the RNA-seq data sets
    Sequencing read mapping to the human reference genome
    Transcript quantification & Data visualization

    Dr. Andreas Mayer: mayer@molgen.mpg.de close

Subjects A - Z