20104901 Vorlesung

SoSe 16: Production of biological samples in biophysics

Ramona Schlesinger

Zusätzl. Angaben / Voraussetzungen

max. 6 Plätze; Nachrückerliste: r.schlesinger@fu-berlin.de

 

Kommentar

Lectures  about how to mutagenise a protein, cloning techniques, expression and purification of proteins will be given prior to the practical exercises in the lab.

Practical exercises:

-molecular biological techniques e.g. transformation of bacteria with plasmids in preparation of expressing a membrane protein

-cultivation of bacteria to express the protein

-purification of membrane proteins by affinity chromatography

-amplification of DNA by PCR (polymerase chain reaction)

-analysis of DNA with enzymes and agarose-gel-electrophoresis and analysis of proteins on SDS-gels.

Schließen

5 Termine

Regelmäßige Termine der Lehrveranstaltung

Mo, 26.09.2016 09:00 - 14:30

Dozenten:
Dr. Ramona Schlesinger

Di, 27.09.2016 09:00 - 14:30

Dozenten:
Dr. Ramona Schlesinger

Mi, 28.09.2016 09:00 - 14:30

Dozenten:
Dr. Ramona Schlesinger

Do, 29.09.2016 09:00 - 14:30

Dozenten:
Dr. Ramona Schlesinger

Fr, 30.09.2016 09:00 - 14:30

Dozenten:
Dr. Ramona Schlesinger

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