216502a
Lecture
SoSe 20: Macromolecular (protein-protein, protein-DNA/RNA) interactions
Sutapa Chakrabarti
Information for students
Powerpoint with voice-over and/or videoconference at the regular time.
The number of participant for this semester has been increased to
25 people. Please register yourselves for this module through Campus
Management and at the same time, send an email to the lecturer
(chakraba@zedat.fu-berlin.de).
More information about the course will be made available through Blackboard.
The class will only be held if at least 6 students are registered on the day before the first lecture! close
The class will only be held if at least 6 students are registered on the day before the first lecture! close
Comments
Inhalt / Content:
Prof. Dr. Sutapa Chakrabarti: chakraba@zedat.fu-berlin.de close
- Thermodynamic and kinetic definitions of macromolecular interactions
- Biochemical methods of determining protein-protein interactions (co-precipitation, size-exclusion analysis, cross-linking mass spectrometry, TAP, FRET, yeast 2-hybrid, proximity-dependent biotin identification)
- Biochemical methods of determining protein nucleic acid interactions (gel-shift assay, protection/foot-printing assays, yeast 3-hybrid)
- Biophysical methods of analysing protein-protein/protein-nucleic acid interactions (analytical ultra-centrifugation, light scattering, isothermal titration calorimetry, surface plasmon resonance, fluorescent anisotropy, microscale thermophoresis)
- Presentation and interpretation of biochemical and biophysical data
- High throughput methods of analysis protein-nucleic acid interactions (micro-array technology, next-generation sequencing based methods such as CLIP and its variations, CRAC, Hits-FLIP and adaptation of classical methods such as SELEX and ChIP to high throughput techniques.
- High throughput methods of analysing protein-protein interactions (computational methods as well as proteomics-based approaches)
Prof. Dr. Sutapa Chakrabarti: chakraba@zedat.fu-berlin.de close